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Ten Commandments of DNA and Polyphosphate Enzymology |
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Lessons learned from forty years of work on DNA replication and the last ten
years on inorganic polyphosphate will be updated in the context of ten THOU
SHALTs:
I. rely on enzymology to clarify biologic questions;
II. Trust the universality of biochemistry and the power of microbiology;
III. do not believe something because you can explain it;
IV. do not waste clean thinking on dirty enzymes;
V. do not waste clean enzymes on dirty substrates;
VI. depend on viruses to open windows;
VII. correct for extract dilution with molecular crowding;
VIII. respect the personality of DNA;
IX. use reverse genetics and genomics;
X. employ enzymes as unique reagents.
These several topics will be presented in a historical and personalized context.
Arthur Kornberg
Beckman Center, B-400
Department of Biochemistry
Stanford University School of Medicine
Stanford, CA 94305-5307 USA |
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Last Updated ( Monday, 01 August 2005 )
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